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CD8 Mouse anti-Human, Brilliant Violet 650, Clone: G42-8, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743067

Catalog No. BDB743067


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Description

Description

The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8ααhomodimer or CD8αβheterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. This antibody is useful in detecting CD8+ T cells when using DimerX I reagents.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Specifications

Specifications

CD8
G42-8
Brilliant Violet 650
CD8α, CD8A, CD8 alpha, Leu2, MAL, T8, p32
Affinity Purified
RUO
Human
Mouse IgG2a κ
Monoclonal
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Mouse
50 μg
Primary
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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