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CD80 (B7-1) Armenian Hamster anti-Canine, Mouse, Porcine, Clone: 16-10A1, eBioscience™ Non-distribution product as customer accommodation.

Armenian Hamster Monoclonal Antibody

Manufacturer:  Invitrogen 14080181

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Catalog No. 50-127-13


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Description

Description

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. 16-10A1 has also been reported in blocking of CD80 to its ligands. (Please use Functional Grade purified 16-10A1, cat. 16-0801, in functional assays.). Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Specifications

Specifications

CD80 (B7-1)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q00609
B7-1, B7.1, Cd28l, Ly-53, Ly53, MIC17, TS/A-1, LAB7, B7.1, CD28LG, CD28LG1
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Mouse, Porcine
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization
16-10A1
Unconjugated
Cd80
Liquid
CD80
IgG
50 μg
4° C
Primary
12519, 397161, 403765
SDS
Documents
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