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CD80 (B7-1) Armenian Hamster anti-Canine, Mouse, Porcine, Functional Grade, Clone: 16-10A1, eBioscience™ Non-distribution product as customer accommodation.

Armenian Hamster Monoclonal Antibody

$172.00 - $306.00

Specifications

Antigen CD80 (B7-1)
Clone 16-10A1
Target Species Canine, Mouse, Porcine
Conjugate Functional Grade
Isotype IgG
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-143-89 Non-distribution product as customer accommodation.
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Invitrogen
16080182
100 μg
Each for $172.00
Add to cart
 
50-143-90 Non-distribution product as customer accommodation.
View Documents
Invitrogen
16080185
500 μg
Each for $306.00
Add to cart
 
Description

Description

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis. 16-10A1 has also been reported in blocking of CD80 to its ligands. Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC. CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Specifications

Specifications

CD80 (B7-1)
Canine, Mouse, Porcine
IgG
Monoclonal
Liquid
Antibody
4° C
16-10A1
Functional Grade
Flow Cytometry, Functional Assay, Neutralization
Primary
Monoclonal
PBS with no preservative; pH 7.2
RUO
SDS

For Research Use Only.

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