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CD80 (B7-1) Monoclonal Antibody (2D10.4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 46080942
Description
Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of Daudi cell line. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.
CD80 (B7-1) Monoclonal antibody specifically detects CD80 (B7-1) in Human samples. It is validated for Flow Cytometry.Specifications
CD80 (B7-1) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P33681 | |
CD80 | |
Affinity chromatography | |
RUO | |
941 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
2D10.4 | |
PerCP-eFluor 710 | |
CD80 | |
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
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