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CD80 (B7-1) Mouse anti-Rat, PE, Clone: 3H5, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$194.00 - $317.00

Specifications

Antigen CD80 (B7-1)
Clone 3H5
Target Species Rat
Conjugate PE
Isotype IgG1, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-104-81 Non-distribution product as customer accommodation.
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Invitrogen
12080082
100 μg
Each for $194.00
Add to cart
 
50-104-82 Non-distribution product as customer accommodation.
View Documents
Invitrogen
12080083
200 μg
Each for $317.00
Add to cart
 
Description

Description

Description: The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ~70 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands. Applications Reported: The 3H5 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Specifications

Specifications

CD80 (B7-1)
Rat
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
3H5
PE
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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