Description: The eBio17G10.2 monoclonal antibody reacts with human immunoglobulin-like transcript 7 (ILT7), also known as CD85g. ILT7 is part of the leukocyte immunoglobulin-like receptor (LIR) gene family and associates with Fc epsilon R1 gamma to form a functional complex which transduces ITAM-mediated signals that negatively modulates TLR-induced type I IFN production by human plasmacytoid dendritic cells. ILT7 is expressed exclusively on plasmacytoid dendritic cells and is absent in monocyte derived DCs. Treatment with IL-3 or CpG causes a decrease in expression. Double staining of human PBMCs with eBio17G10.2 and anti-pDC marker BDCA2 yields virtually complete co-staining. Cross linking of ILT7 with eBio17G10.2 antibody causes reduced transcription of IFN alpha1, IFN alpha2, IFN alpha4 and IFN beta. Applications Reported: This eBio17G10.2 (17G10.2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio17G10.2 (17G10.2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.This gene encodes an immunoglobulin-like cell surface protein preferentially expressed in plasmacytoid dendritic cells (PDCs). This gene is highly expressed in PDCs, and is found to be rapidly down-regulated by interleukin 3 (IL3). This gene is one of the 19 highly related genes that form a leukocyte receptor gene cluster (LRC) at chromosomal region 19q13.4.
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
For Research Use Only.
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