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CD85h (ILT1) Rat anti-Human, PE, Clone: 135.4, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 12511942

Encompass

Catalog No. 50-108-02



Description

Description

Description: The monoclonal antibody 135.4 recognizes ILT1, also known as CD85h, LIR7 and LILRA2. ILT1 is a member of the large family of ILT molecules (Immunologulin-like Transcripts) also known as Leukocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT1 contains a short cytoplasmic domain. Through the cytoplasmic domain ILT1 associates with the Fc receptor gamma chain but not DAP12 resulting in a stimulatory signal. ILT1 may act as a receptor for MHC Class I antigens. Expression of ILT1 is mainly found on monocytes, macrophages, DC and weakly on granulocytes. ILT1 is thought to play a role in the TH1/Th2 balance as well as TLR signaling. The monoclonal antibody 135.4 has been reported to have activating properties upon crosslinking which result in calcium influx. Applications Reported: This 135.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 135.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Leukocyte Ig-like receptors (LIRs) are a family of immunoreceptors expressed predomitly on monocytes and B cells and at lower levels on dendritic cells and natural killer (NK) cells. All LIRs in subfamily B have an inhibitory function (see,e.g., LILRB1, MIM 604811). LIRs in subfamily A, with short cytoplasmic domains lacking an immunoreceptor tyrosine-based inhibitory motif (ITIM) and with trans membrane regions containing a charged arginine residue, may initiate stimulatory cascades. One member of subfamily A (LILRA3; MIM 604818) lacks a transmembrane region and is presumed to be a soluble receptor.
Specifications

Specifications

CD85h (ILT1)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q8N149
CD85H, ILT1, LIR-7, LIR7, CD85 antigen-like family member H, ILT-1, immunoglobulin-like transcript 1, leukocyte immunoglobulin-like receptor 7, leukocyte immunoglobulin-like receptor subfamily A member 2, leukocyte immunoglobulin-like receptor subfamily A member 2 soluble
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
135.4
PE
LILRA2
Liquid
LILRA2
IgG2a, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
11027
SDS
Documents
Product Certifications
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