CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor™ Yellow 690, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES H021T02Y05

Catalog No. H021T02Y05

Description

The IT2.2 monoclonal antibody reacts with human CD86, an ∽80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response. Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.2 μg) per test. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Excitation: 552 nm; Emission: 690 nm; Laser: 561 nm (Yellow) Laser NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts.

CD86 (B7-2) Monoclonal antibody specifically detects CD86 (B7-2) in Human samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD86 (B7-2)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P42081
• Gene Symbols: CD86
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 942
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: IT2.2
• Conjugate: NovaFluor Yellow 690
• Gene: CD86
• Gene Alias: activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ