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CD86 (B7-2) Monoclonal Antibody (GL1), PE-Cyanine7, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 25086282
Description
Description: The GL1 monoclonal antibody reacts with mouse CD86, an ∽80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response. Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
CD86 (B7-2) Monoclonal antibody specifically detects CD86 (B7-2) in Mouse samples. It is validated for Flow Cytometry.Specifications
CD86 (B7-2) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P42082 | |
CD86 | |
Affinity chromatography | |
RUO | |
12524 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
GL1 | |
PE-Cyanine7 | |
CD86 | |
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2 | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
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For Research Use Only.