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CD8a Rat anti-Mouse, Biotin, Clone: 4SM15, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 13080880

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Catalog No. 50-112-2516


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Description

Description

Description: The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. Recent studies have shown the 4SM15 antibody recognizes and binds a non-CD8a epitope in neural tissue. The 4SM15 clone is not recommended for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com). For detection of mouse CD8a in FFPE mouse neural tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no 14-0195). The 4SM15 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD8a using immunohistochemistry of frozen tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no. 14-0081). Applications Reported: This 4SM15 antibody has been reported for use in flow cytometric analysis, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This 4SM15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. The 4SM15 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered. CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).
Specifications

Specifications

CD8a
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01731
CD8, Leu-2, MAL, p32, CD8a
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry, Immunohistochemistry (Paraffin)
4SM15
Biotin
Cd8a
Liquid
Cd8a
IgG2a, lambda
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12525
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