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CD8b Mouse anti-Human, Non-human primate, PerCP-eFluor™ 710, Clone: SIDI8BEE, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  eBioscience 46527341

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Catalog No. 46-527-341


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Description

Description

The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta. The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
Specifications

Specifications

CD8b
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P10966
CD8B
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
926
Flow Cytometry
SIDI8BEE
PerCP-eFluor™ 710
CD8B
Liquid
Mouse
Affinity Chromatography
RUO
Antibody
Monoclonal
Human, Non-human primate
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