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CD9 Rat anti-Mouse, eFluor® 450, Clone: eBioKMC8 (KMC8), eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 48009182

Catalog No. 50-112-4088


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Description

Description

Description: The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on naive T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability. Applications Reported: This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test.

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered. CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.
Specifications

Specifications

CD9
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P40240
5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, MRP-1, P24, TSPAN-29
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
eBioKMC8 (KMC8)
eFluor™ 450
Cd9
Liquid
Cd9
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12527
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