CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright™ 645, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
64-090-082	Invitrogen™ 64090082	100 μg

Description

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Specifications

• Antigen: CD90.1 (Thy-1.1)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P01830, P01831
• Gene Symbols: Thy1
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Thy1

• Clone: HIS51
• Applications: Flow Cytometry
• Conjugate: Super Bright 645
• Host Species: Mouse
• Target Species: Mouse, Rat
• Gene ID (Entrez): 21838, 24832
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody