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CD95 (APO-1/Fas) Mouse anti-Canine, Human, Clone: DX2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$94.25 - $181.00

Specifications

Antigen CD95 (APO-1/Fas)
Clone DX2
Target Species Canine, Human
Conjugate Unconjugated
Isotype IgG1, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-127-66 Non-distribution product as customer accommodation.
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Invitrogen
14095980
25 μg Each for $94.25
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50-127-67 Non-distribution product as customer accommodation.
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Invitrogen
14095982
100 μg Each for $181.00
Add to cart
 
Description

Description

Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95. Applications Reported: The DX2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. DX2 has also been reported in in vitro studies of Fas-induced apoptosis. EOS9.1 is recommended for induction of apoptosis in vitro . Applications Tested: The DX2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Specifications

Specifications

CD95 (APO-1/Fas)
Canine, Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
DX2
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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