Invitrogen™ CD99 Monoclonal Antibody (3B2/TA8), APC, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
17-099-742	Invitrogen™ 17099742	100 Tests

Description

Description: The 3B2/TA8 monoclonal antibody reacts with human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed highly by all leukocytes. However, there is little to no expression in granulocytes. CD99 is involved in T cell adhesion and activation. Applications Reported: This 3B2/T8A antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3B2/T8A antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Specifications

• Antigen: CD99
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P14209
• Gene ID (Entrez): 4267
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 3B2/TA8
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human
• Gene: CD99
• Gene Alias: 1110061M03Rik; 12E7; 2410026K10Rik; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; Cd99; CD99 antigen; CD99 molecule; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; D4; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; paired immunoglobin-like type 2 receptor-ligand; pilr-1; Pilrl; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2
• Gene Symbols: CD99
• Product Type: Antibody