CEACAM-5/CD66e Mouse anti-Human, Clone: COL-1 + CEA31 + C66/261, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234233F	Novus Biologicals NBP2342330.02MG	0.02 mg
NBP234233A	Novus Biologicals NBP2342330.1MG	0.1 mg
NBP234233B	Novus Biologicals NBP2342330.2MG	0.2 mg

Description

CEACAM5/CD66e Monoclonal specifically detects CEACAM5/CD66e in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CEACAM5/CD66e
• Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 1-2ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P06731
• Gene ID (Entrez): 1048
• Immunogen: Human colon carcinoma extract (COL-1 & CEA31); CEA recombinant protein (C66/261)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: COL-1 + CEA31 + C66/261
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cellular Markers, Immunology
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100
• Gene Symbols: CEACAM5
• Isotype: IgG2a, IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.