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Invitrogen™ Chargeswitch™ Pro Filter Plasmid Maxi Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Chargeswitch™ Pro Filter Plasmid Maxi Kit

$411.00 - $740.00

Specifications

Final Product Type Pro Filter Plasmid Maxi Kit
For Use With (Application) Cloning, in vitro transcription, next-generation sequencing, nucleic acid labeling, PCR, sequencing, transfection, transformation
Includes The ChargeSwitch™-pro plasmid Kit contains columns with resuspension buffer, RNase A, lysis buffer, precipitation buffer, wash buffers 1 and 2, and elution buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored
Isolation Technology ChargeSwitch™ chemistry
Packaging Type 1 Kit(s)
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
CS31106 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. View Documents Invitrogen™
CS31106
10 Prep(s) Each for $411.00
Add to cart
 
CS31107 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. View Documents Invitrogen™
CS31107
25 Prep(s) Each for $740.00
Add to cart
 
Description

Description

The Chargeswitch™ -Pro Filter Plasmid Maxi Kit provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.



To accomplish this, the ChargeSwitch™-Pro Filter Kit provides:

  • 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
  • Cleaner purified product for improved performance in downstream applications
  • A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
  • Endotoxin units < 10 EU ⁄ ug DNA for
  • Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)



    How it works
    The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

  • Specifications

    Specifications

    Pro Filter Plasmid Maxi Kit
    The ChargeSwitch™-pro plasmid Kit contains columns with resuspension buffer, RNase A, lysis buffer, precipitation buffer, wash buffers 1 and 2, and elution buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored
    1 Kit(s)
    Bacteria general, bacterial culture specific
    800μg
    Pro Filter Plasmid Maxi Kit
    Cloning, in vitro transcription, next-generation sequencing, nucleic acid labeling, PCR, sequencing, transfection, transformation
    ChargeSwitch™ chemistry
    Transfection grade
    Room temperature
    Spin column

    For Research Use Only. Not for use in diagnostic procedures.

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