Invitrogen™ Chk1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA596749

Catalog No. PIPA596749

Description

Positive Samples: SW620, HT-29, Mouse thymus, Mouse testis, Rat testis; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center Immunogen sequence: DFGLATVFRY NNRERLLNKM CGTLPYVAPE LLKRREFHAE PVDVWSCGIV LTAMLAGELP WDQPSDSCQE YSDWKEKKTY LNPWKKIDSA PLALLHKILV ENPSARITIP DIKKDRWYNK PLKKGAKRPR VTSGGVSESP SGFSKHIQSN LDFSPVNSAS.

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

Specifications

• Antigen: Chk1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Chek1
• Gene Alias: C85740; Cell cycle checkpoint kinase; checkpoint kinase 1; checkpoint kinase 1 homolog; checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27; rad27 homolog; serine/threonine protein kinase CHK1; serine/threonine-protein kinase Chk1; Synoviolin; YBR1742; YBR274W
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1111, 12649, 140583
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1.35 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Gene Accession No.: O14757, O35280, Q91ZN7
• Gene Symbols: Chek1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 148-307 of Chk1 (NP_001265.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG