Chromogranin A Mouse anti-Human, Primate, Mouse, Rat, Alexa Fluor™ 350, Clone: PHE5, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP234709AF350

Catalog No. NP234709A30

Description

Chromogranin A Monoclonal specifically detects Chromogranin A in Human, Primate, Monkey, Mouse (Negative), Rat (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Chromogranin A
• Clone: PHE5
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: betagranin (N-terminal fragment of chromogranin A), CGA, chromogranin A (parathyroid secretory protein 1), chromogranin-A, parathyroid secretory protein 1, Pituitary secretory protein I, SP-I
• Host Species: Mouse
• Isotype: IgG1 Kappa
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Gene ID (Entrez): 1113
• Target Species: Human, Primate, Mouse, Rat

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Conjugate: Alexa Fluor 350
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: CHGA
• Immunogen: Human pheochromocytoma
• Purification Method: Protein A or G purified
• Storage Requirements: Store at 4°C in the dark.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.