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Novus Biologicals™ Coagulation Factor X Antibody Pair [HRP]

Monoclonal Antibody Pair

Manufacturer:  Novus Biologicals™ NBP27934215PLATES

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Catalog No. NB009723


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Description

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Coagulation Factor X.
Specifications

Specifications

Sandwich ELISA
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Coagulation Factor X.
5.47 pg/mL
2159
RUO
Coagulation Factor X
15 Plates
5.47-350 pg/mL
coagulation factor X, EC 3.4.21, EC 3.4.21.6, factor Xa, FX, FXA, prothrombinase, Stuart factor, Stuart-Prower factor
HRP
Antibody Pairs
Sandwich ELISA
  • Standard: Each vial contains 30 ng of recombinant Human Coagulation Factor X.
  • Capture Antibody: 1 mg/mL of mouse anti-Human Coagulation Factor X monoclonal antibody (in PBS, pH 7.4).
  • Detection Antibody: 0.2 mg/mL of rabbit anti-Human Coagulation Factor X monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Storage is content dependent.
    Rabbit/Mouse
    Human
    F10
    1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 350 pg/mL is recommended.
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