Complement C5a Monoclonal Antibody (2942), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA140161

Catalog No. 01-676-534

Description

MA1-40161 detects C5a/C5a des-Arg from human samples. MA1-40161 has been successfully used in Western blot, ELISA, immunohistochemistry (frozen), and functional assay applications. By Western blot, the antibody detects a band size of 14 kDa. The MA1-40161 immunogen is the Neo-epitope on C5a. Positive control: Eye tissue of patient with AMD; Negative control: Eye tissue of patient without AMD. Endotoxin <24 EU/mg. General comment: Please notice that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products.

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.

Specifications

• Antigen: Complement C5a
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Gene Accession No.: P01031
• Gene Symbols: C5
• Immunogen: Neo-epitope on C5a.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: ELISA, Functional Assay, Immunohistochemistry (Frozen), Western Blot
• Clone: 2942
• Conjugate: Unconjugated
• Gene: C5
• Gene Alias: anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; C5; C5a; C5a anaphylatoxin; C5b; C5D; CH; CO4; complement C5; Complement C5 alpha chain; Complement C5 alpha' chain; Complement C5 beta chain; complement component 5; CPAMD3; CPAMD4; DADB-112B14.11; ECLZB; FLJ17816; FLJ17822; FLJ60561; Hc; He; Hemolytic complement; MGC142298; MGC164979; prepro-C5; RGD1561905
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 727
• Content And Storage: 4° C
• Form: Liquid