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Invitrogen™ Complement Factor D/Adipsin Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHCFD

Encompass

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Catalog No. EHCFD


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Description

Description

The Human Complement Factor D (Adipsin/CFD) ELISA quantitates Hu Complement Factor D in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Complement Factor D. Principle of the method The Human Complement Factor D solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.
Specifications

Specifications

Colorimetric Microplate Reader
96 Assays
Biotin
TMB
HRP
8.23-6000 pg/mL
CFD (Adipsin) ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Other Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Cfd (Adipsin) in Samples, Using a Microplate Reader
1675
RUO
2°C to 8°C
4 hr. 45 min.
CFD (Adipsin)
Other Research
<12%
96 Tests
Plasma, Serum, Supernatant
ADIPSIN,ADN,DF,PFD
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
ELISA Kit
Solid-phase sandwich ELISA
Other Proteins
P00746
4pg/mL to 6000pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Human CFD precoated 96-well strip plate, lyophilized recombinant human CFD standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent A, contains 0.09%
Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
Human
ADIPSIN, ADN, DF, PFD
<10%
Human
4 pg/mL
Colorimetric
SDS
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Product Certifications
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