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Invitrogen™ Complement Factor D/Adipsin Human ELISA Kit
Sandwich ELISA Kit
$598.00 - $4840.00
Specifications
Accession Number | P00746 |
---|---|
Assay Sensitivity | 4 pg/mL |
Description | CFD (Adipsin) ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Other Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Cfd (Adipsin) in Samples, Using a Microplate Reader |
Detectable Analytes | CFD (Adipsin) |
Assay | Solid-phase sandwich ELISA |
Description
The Human Complement Factor D (Adipsin/CFD) ELISA quantitates Hu Complement Factor D in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Complement Factor D. Principle of the method The Human Complement Factor D solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.Specifications
P00746 | |
CFD (Adipsin) ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Other Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Cfd (Adipsin) in Samples, Using a Microplate Reader | |
Solid-phase sandwich ELISA | |
Colorimetric | |
Other Proteins | |
TMB | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
1675 | |
Human | |
<10% | |
RUO | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
4 pg/mL | |
CFD (Adipsin) | |
Biotin | |
ELISA Kit | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF | |
Human | |
Colorimetric Microplate Reader | |
ADIPSIN,ADN,DF,PFD | |
<12% | |
HRP | |
Other Research | |
ADIPSIN, ADN, DF, PFD | |
1 hr. 20 min. |
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