Novus Biologicals™ Complement Factor H Antibody Pair [HRP]

Mouse Monoclonal Antibody Pair

Supplier:  Novus Biologicals™ NBP27952015PLATES

Catalog No. NB010079

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Complement Factor H.

Specifications

• Assay Range: 46.88 pg/mL
• Assay: Sandwich ELISA
• Product Type: Antibody Pairs
• Target Species: Human
• Gene Alias: adrenomedullin binding protein, age-related maculopathy susceptibility 1, AHUS1, AMBP1, ARMD4, beta-1H, beta-1-H-globulin, CFHL3, complement factor H, factor H, factor H-like 1, FH, FHL1, H factor 1, H factor 1 (complement), H factor 2 (complement), HF, HF1ARMS1, HF2, HUSMGC88246
• Gene Symbol: CFH
• Kit Contents:
• Standard: Each vial contains 38 ng of recombinant Human Complement Factor H.
• Capture Antibody: 0.5 mg/mL of mouse anti-Human Complement Factor H monoclonal antibody (in PBS, pH 7.4).
• Detection Antibody: 0.2 mg/mL of mouse anti-Human Complement Factor H monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Regulatory Status: RUO
• Target: Complement Factor H

• Assay Sensitivity: 46.88-3000 pg/mL
• Conjugate: HRP
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Complement Factor H.
• For Use With (Application): Sandwich ELISA
• Gene ID (Entrez): 3075
• Host Species: Mouse
• Quantity: 15 Plates
• Storage Requirements: Storage is content dependent.
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C immediately (in 15 mins) in a manual defrost freezer, otherwise the value could decrease significantly. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.