BioVision Inc EZExtract Core Histone Isolation Kit: Histone Isolation Kit: Simple method to isolate histones with minimal sample preparation. 100 Extractions
Manufacturer: BioVision Inc K234100
EZExtract Core Histone Isolation Kit: Histones are small alkaline proteins that interact with eukaryotic DNA to form the fundamental subunit of chromatin known as the nucleosome. The core histones H2A, H2B, H3 and H4 form the central spooling unit of the nucleosome; with two copies of each comprising a histone octamer that interacts with the negatively charged DNA. Post-translational modification of this core complex results in structural changes within the chromatin and epigenetic control of gene expression. BioVision's EZExtract Core Histone Isolation Kit offers an optimized suite of reagents to rapidly extract the total core histones while preserving acetylation from tissues and cultured cells for biochemical and epigenetic analyses. One hundred standard extractions at a scale of 107 cells or 100 mg tissues can be performed with the reagents in this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue
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