Combines the fluorescence blocking capabilities of the BD Falcon FluoroBlok membrane with the benefits of an extracellular matrix protein
Provides a predictive in vitro model for screening pro- and anti-angiogenic agents
Eliminates tedious manual cell scraping and counting
Fibronectin concentration and coating procedure have been optimized so the pores of the membrane are not obstructed, allowing endothelial cells to attach to the membrane and freely migrate toward an angiogenic stimulus in the receiver plate
Cells may be labeled with a fluorescent dye either pre- or post-migration and quantified using a fluorescence microplate reader
Light-tight membrane blocks the transmission of light within the 490 to 700nm range, shielding fluorescently labeled cells in the top of the insert from detection by a bottom-reading microplate reader and allowing for real-time detection without dismantling the insert
Tested for the ability to support migration of HUVEC (human umbilical vein endothelial cells) in response to VEGF (vascular endothelial cell growth factor)
Stable for at least three months when stored at -20°C
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