COX2 Monoclonal Antibody (COX 229), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
48-972-582	LIFE TECHNOLOGIES 48972582	100 μg

Description

Description: This COX 229 monoclonal antibody recognizes human COX2 also known as PTGS2. This antibody has been reported to cross-react with the mouse and rat COX2. For optimal performance we recommend using this antibody with the Foxp3 / Transcription Factor Staining Buffer Set and protocol. This antibody is predicted to cross-react with the mouse and rat homologue. Applications Reported: This COX 229 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This COX 229 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at www.thermofisher.com/flowprotocols. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor 450 is an alternative to Pacific Blue.

eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

Specifications

• Antigen: COX2
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: PTGS2, prostaglandin-endoperoxide synthase 2, Cyclooxygenase-2, PHS II, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
• Gene Symbols: PTGS2
• Isotype: IgG1 κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: COX 229
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P35354
• Gene ID (Entrez): 5743
• Immunogen: Synthetic peptide from human COX2
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: PTGS2