CRISP-1 Rat anti-Mouse, Clone: 840017, R&D Systems™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MAB4675	R&D Systems MAB4675	100 ug
MAB4675SP	R&D Systems MAB4675SP	25 ug

Description

Cysteine-rich Secretory Protein 1 (CRISP-1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm-Coating Glycoprotein 1 (SCP1), is a 29-32kDa (26.4kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N-terminal SCP/CAP domain, a hinge region, and a Cys-rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP-1 in mouse and rat is CRISP-4 (5, 6). Mature mouse CRISP-1 shares 68% amino acid (aa) sequence identity with rat CRISP-1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP-2, -3, and -4, respectively. Rodent CRISP-1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7-9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP-1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP-1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13-15).

Specifications

• Antigen: CRISP-1
• Format: Purified
• Applications: Immunohistochemistry, Neutralization, Western Blot
• Host Species: Rat
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO

• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Clone: 840017
• Conjugate: Unconjugated
• Isotype: IgG2b
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.