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CXCL9 (MIG) Armenian Hamster anti-Mouse, eFluor® 660, Clone: MIG-2F5.5, eBioscience™ Non-distribution product as customer accommodation.

Armenian Hamster Monoclonal Antibody

Manufacturer:  Invitrogen 50300980

Catalog No. 50-112-8955



Description

Description

Description: The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN gamma (mig, mig-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN gamma, but not other activators such as LPS or IFN alpha. Secretion of mig, mainly by macrophages results in the chemotaxis of a variety of activated T cells via the CXCR3 chemokine receptor. Mig is involved many areas of research including autoimmune diseases, cancer, and inflammation. Applications Reported: This MIG-2F5.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MIG-2F5.5 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated RAW 264.7 cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.

2 µm post-manufacturing filtered. MIG belongs to a family of chemoattractant cytokines that play a role in monocyte, and T lymphocyte chemoattraction. MIG is expressed in monocytes/macrophages, hepatocytes, fibroblasts, keratinocytes, and endothelial cells in response to interferon-gamma. MIG is related functionally to interferon-inducible protein 10 (IP10), as both bind to the CXCR3 receptor to inhibit endothelial cell chemotaxis, and growth factor-induced angiogenesis.
Specifications

Specifications

CXCL9 (MIG)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P18340
CMK, Humig, MIG, SCYB9, crg-10
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
MIG-2F5.5
eFluor™ 660
Cxcl9
Liquid
Cxcl9
IgG
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
17329
Documents
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