Mouse anti-Cyclin B1, Clone: SPM619, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP2446880.02MG
Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), ImmunofluorescenceCyclin B1 Monoclonal antibody specifically detects Cyclin B1 in Human, Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
|10mM PBS and 0.05% BSA with 0.05% Sodium Azide|
|CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1|
|Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors|
|Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence|
|Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml|
|Recombinant human full-length CCNB1 protein|
|Protein A or G purified|
|Store at 4C.|
|It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.|
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