Cyclin D1 Mouse anti-Human, Clone: CCND1/809, Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2479210.1MG

Catalog No. NBP247921A

Description

Cyclin D1 Monoclonal specifically detects Cyclin D1 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.

Specifications

• Antigen: Cyclin D1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1D11S287E, cyclin D1, cyclin D1 (PRAD1: parathyroid adenomatosis 1), G1/S-specific cyclin D1, G1/S-specific cyclin-D1, PRAD1 oncogene, PRAD1B-cell CLL/lymphoma 1, U21B31
• Host Species: Mouse
• Molecular Weight of Antigen: 36 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cell Cycle and Replication, Core ESC Like Genes, mTOR Pathway, Stem Cell Markers, Wnt Signaling Pathway
• Gene ID (Entrez): 595
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: CCND1/809
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: CCND1
• Immunogen: Recombinant human CCND1 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ