Invitrogen™ Cyclin G1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536050

Catalog No. PIPA536050

Description

This antibody detects endogenous protein at a molecular weight of 29 kDa. Purity is >95% by SDS-PAGE.

Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. The Cdc2- cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression. The G1 to S transition, however, appears to be controlled by the G1 cyclins. Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.

Specifications

• Antigen: Cyclin G1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ccng1
• Gene Alias: AI314029; CCNG; CCNG1; CYCG; CYCG1; cyclin G1; cyclin-G; Cyclin-G1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12450, 25405, 900
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P39950, P51945, P51959
• Gene Symbols: Ccng1
• Immunogen: Synthetic peptide corresponding to amino acids 180-220 of human Cyclin G.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG