BioVision Inc Cytochrome P450 1A2 (CYP1A2) Activity Assay Kit (Fluorometric): Highly Sensitive; Includes human CYP1A2 - NADPH Reductase as positive control.
Manufacturer: BioVision Inc K893100
Cytochrome P450 1A2 (CYP1A2) Activity Assay Kit (Fluorometric): Cytochrome P450 1A2 (CYP1A2, EC 188.8.131.52) is a member of the cytochrome P450 monooxidase (CYP) family of microsomal xenobiotic metabolism enzymes. BioVision's CYP1A2 Activity Assay Kit enables rapid measurement of native or recombinant CYP1A2 activity in biological samples such as liver microsomes. The assay utilizes a non-fluorescent CYP1A2 substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 406/468 nm). The inhibitor displays greater than 20-fold selectivity for CYP1A2 over other CYPs, ensuring targeted inhibition. CYP1A2 specific activity is calculated by running parallel reactions in the presence and absence of the selective inhibitor and subtracting any residual activity detected with the inhibitor present. The kit contains a complete set of reagents sufficient for performing 50 sets of paired reactions (in the presence and absence of inhibitor).
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