BioVision Inc Cytochrome c Releasing Apoptosis Assay Kit: Cytochrome c Releasing Apoptosis Assay Kit: Convenient & Effective Assay for Detecting Cytochrome c Translocation from Mitochondria into Cytosol during Apoptosis. No need to Ultracentrifuge. 100 Assays.
Manufacturer: BioVision Inc K257100
Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. BioVision’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. BioVision charges an Ice pack fee for each order.
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