BioVision Inc Cytochrome P450 Reductase (CPR) Activity Kit (Colorimetric): Quantitative 96-well plate assay of cytochrome P450 reductase activity in tissues, cells, microsomes or purified CPR enzyme. Simple & Sensitive. Detection limit: ~0.2 mU per well. 100 wells
Manufacturer: BioVision Inc K700100
Cytochrome P450 Reductase (CPR) Activity Kit (Colorimetric): NADPH-cytochrome P450 reductase (CPR, EC 22.214.171.124) is a ~78 kDa membrane-bound flavoenzyme that catalyzes the transfer of electrons from NADPH to members of the cytochrome P450 monooxidase (CYP) enzyme family in the endoplasmic reticulum. BioVision's cytochrome P450 reductase activity assay kit couples oxidation of NADPH by CPR to reduction of a nearly colorless probe into a brightly colored product with an absorbance peak at 460 nm, with the rate of color generation being directly proportional to CPR activity. The kit can be used to determine CPR activity in a variety of samples, with a detection limit of ~0.2 mU of CPR activity per reaction. For assessment of CPR activity in crude biological samples that may have extraneous reductases capable of reducing the substrate, an inhibitor of NADPH-dependent flavoproteins is included. The kit contains sufficient reagents for performing 100 reactions in a 96-well plate format.
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