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Cytochrome C Mouse anti-Human, Mouse, Rat, FITC, Clone: 6H2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 11660182

Catalog No. 50-997-3

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Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c. Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis. Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.


Cytochrome c
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P99999, P62897, P62898
cyt c
Affinity chromatography
Human, Mouse, Rat
Flow Cytometry, Immunocytochemistry, Immunofluorescence
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
13063, 25309, 54205
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