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Cytokeratin 10/13 Mouse anti-Canine, Feline, Human, Clone: EBS-IF-003, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA533317

Catalog No. PIMA533317

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MA5-33317 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections MA5-33317 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections MA5-33317 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.

Cytokeratins(CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. Skin) and non-keratinizing cells (ie. Mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different CKs in the molecular range of 40-70 k Da and isoelectric points of 5-805 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.


Cytokeratin 10/13
125 μg/mL
PBS with 0.02% sodium azide
P13645, P13646, Q6EIZ0
KRT10, KRT13, LOC101087886
Cytoskeleton preparation from HeLa cells
Protein A/G
Canine, Feline, Human
Flow Cytometry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
IgG2a, kappa
100 μg
4° C, do not freeze
101085587, 101087886, 3858, 3860, 490983, 491006
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