Cytokeratin 10 Mouse anti-Human, Alexa Fluor™ 350, Clone: KRT10/844 + KRT10/1275, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP254327AF350

Catalog No. NP24327A30

Description

Cytokeratin 10 Monoclonal specifically detects Cytokeratin 10 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 10
• Clone: KRT10/844 + KRT10/1275
• Dilution: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin
• Format: Purified, Conjugated
• Gene Symbols: KRT10
• Immunogen: Recombinant human KRT10 protein
• Purification Method: Protein A or G purified
• Research Discipline: Cytoskeleton Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 3858.0
• Target Species: Human

• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Conjugate: Alexa Fluor 350
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Alias: BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP
• Host Species: Mouse
• Isotype: IgG1 Kappa
• Quantity: 0.1 mL
• Storage Requirements: Store at 4°C in the dark.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.