Cytokeratin 10 Antibody (KRT10/844 + KRT10/1275) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP254327200UG

Catalog No. NB254327200

Description

Cytokeratin 10 Monoclonal antibody specifically detects Cytokeratin 10 in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, CyTOF-ready.

Specifications

• Antigen: Cytokeratin 10
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, CyTOF-ready
• Gene Alias: BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP
• Host Species: Mouse
• Molecular Weight of Antigen: 56.5 kDa
• Quantity: 200 μg
• Research Discipline: Cytoskeleton Markers
• Gene ID (Entrez): 3858
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
• Clone: KRT10/844 + KRT10/1275
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: KRT10
• Immunogen: Recombinant human KRT10 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
• Content And Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only