Cytokeratin 18 Antibody (DC10), Alexa Fluor™ 532, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP233082AF532

Catalog No. NP233082A32

Description

Cytokeratin 18 Monoclonal specifically detects Cytokeratin 18 in Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 18
• Classification: Monoclonal
• Conjugate: Alexa Fluor 532
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: KRT18
• Immunogen: Human breast cancer PMC 42 cells were used as immunogen to generate the Keratin 18 antibody.
• Quantity: 0.1 mL
• Gene ID (Entrez): 3875
• Target Species: Human, Bovine, Canine, Hamster, Mouse, Porcine, Rat, Sheep
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: DC10
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Content And Storage: Store at 4°C in the dark.