Cytokeratin 5/8 Antibody (SPM268) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2478230.2MG

Catalog No. NBP247823B

Description

Cytokeratin 5/8 Monoclonal antibody specifically detects Antigen in Canine, Chicken (Negative), Ferret, Hamster, Human, Mouse, Porcine, Primate, Rat, Sheep, Xenopus (Negative) samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting.

Specifications

• Antigen: Cytokeratin 5/8
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Gene Alias: cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 3852
• Target Species: Human, Mouse, Rat, Porcine, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: SPM268
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: KRT5
• Immunogen: Cytoskeletal preparation from HeLa cells
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ