Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2479430.1MG

Catalog No. NBP247943A

Description

Cytokeratin 7 Monoclonal specifically detects Cytokeratin 7 in Human, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: Cytokeratin 7
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Gene Alias: CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7
• Host Species: Mouse
• Molecular Weight of Antigen: 55 kDa
• Quantity: 0.1 mg
• Research Discipline: Cytoskeleton Markers
• Gene ID (Entrez): 3855
• Target Species: Human, Rat
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: KRT7/760 + OV-TL12/30
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: KRT7
• Immunogen: Recombinant full-length human KRT7 protein (KRT7/760); OTN 11, ovarian carcinoma cell line (OV-TL12/30)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG