Cytokeratin 8 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA137987

Catalog No. 01-673-632

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung, skin, colon or breast carcinoma.

Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.

Specifications

• Antigen: Cytokeratin 8
• Classification: Polyclonal
• Gene: KRT8
• Gene Alias: CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, cytokeratin 8, cytokeratin-8, keratin, type II cytoskeletal 8, type-II keratin Kb8
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3856
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: P05787
• Gene Symbols: KRT8
• Immunogen: Recombit protein encoding human Keratin 8 1-483aa
• Quantity: 1 mL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG