Invitrogen™ Cytokeratin Pan Type I Monoclonal Antibody (AE1)

Catalog No.	Quantity
01-675-180	200 μL

Catalog No. 01-675-180 Supplier Invitrogen™ Supplier No. MA15761

Description

Mouse Monoclonal Antibody

MA1-5761 detects most human acidic (type I) keratins K10, K14, K15, K16, K19. MA1-5761 has been successfully used in Western blot, immunomicroscopy and immunohistochemistry (paraffin) procedures. For Western blotting, do not use milk for blocking nonspecific binding because it contains some keratins (bovine serum albumin is recommended). The MA1-5761 immunogen is human epidermal keratins.

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Specifications

• Antigen: Cytokeratin Pan Type I
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: AE1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% sodium azide
• Gene: KRT16
• Gene Accession No.: O93256, P02533, P02535, P05783, P05784, P05785, P06394, P08727, P08728, P08779, P13645, P19001, P19012, P35527, Q5BJY9, Q61414, Q61781, Q63279, Q6IFV1, Q6IFV3, Q6IFW6, Q6PVZ1, Q6RHW0, Q8CIS9, Q9Z2K1
• Gene Alias: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; 67 kDa cytokeratin; AA960620; Acidic Cytokeratin; AI324768; AI528832; AI626930; AI663979; AL022697; alpha keratin; AU019895; BCIE; BIE; Card2; Cell proliferation-inducing gene 46 protein; CK1; CK-1; CK10; CK-10; CK14; CK-14; CK15; CK-15; CK16; CK-16; CK18; CK-18; CK19; CK-19; CK8; CK-8; CK-9; CYK18; CYK8; CYKER; cytokeratin 1; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 8; cytokeratin 8 (370 AA); cytokeratin 9; cytokeratin endo A; Cytokeratin endo B; cytokeratin VIB; cytokeratin VII; Cytokeratin-1; Cytokeratin-10; cytokeratin-14; Cytokeratin-15; cytokeratin-16; Cytokeratin-18; cytokeratin-19; Cytokeratin-6B; cytokeratin-7; cytokeratin-8; Cytokeratin-9; cytokeratin-A; D130054E02Rik; EBS3; EBS4; EGK_03685; EHK; EHK1; Endo B; EndoA; EndoC; epidermal keratin 10; epidermal keratin VII; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epidermolytic hyperkeratosis 1; epidermolytic hyperkeratosis; keratosis palmaris et plantaris; epithelial keratin 1; epithelial keratin 10; Epithelial keratin-1; EPPK; FNEPPK; focal non-epidermolytic palmoplantar keratoderma; GK-19; Hair alpha protein; HMWCK; intermediate filament protein; K1; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K2C8; K8; K9; Ka10; Ka14; Ka15; Ka16; Ka19; Kb1; KER1; Ker10; KERA; keratin 1; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 10; keratin 10 (epidermolytic hyperkeratosis); keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 15; keratin 15, type I; keratin 16; keratin 16 (focal non-epidermolytic palmoplantar keratoderma); keratin 16, type I; keratin 18; keratin 18, type I; keratin 19; keratin 19, type I; keratin 24; keratin 8; keratin 8, type II; keratin 86; keratin 9; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 8; keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; keratin, type I cytoskeletal 10; keratin, type I cytoskeletal 10-like; keratin, type I cytoskeletal 13; keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 16; keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; keratin, type I cytoskeletal 59 kDa; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 8; keratin-1; Keratin-10; keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; keratin-16; Keratin-18; keratin-19; Keratin-8; keratin-9; Kerd; KO; KPP; KRT1; krt1 {ECO:0000250; Krt-1.10; Krt-1.14; Krt-1.19; KRT10; krt10 {ECO:0000250; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; KRT13; KRT14; Krt15; krt15 {ECO:0000250; Krt16; KRT16A; KRT18; krt18 {ECO:0000250; KRT19; Krt1-9; KRT1A; Krt-2.1; Krt-2.8; Krt2-1; KRT24; Krt2-8; Krt8; Krt86; krt9; KRTA; KRTB; LOW QUALITY PROTEIN: keratin, type I cytoskeletal 15; NEPPK; NFJ; pan keratin; pankeratin; PC1; PIG46; Spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I alpha-keratin 14; type I alpha-keratin 15; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; Type I keratin Ka14; type I keratin KA15; type I keratin KA16; Type I keratin Ka19; type II cytokeratin A (no.8); type II keratin Kb1; type-II keratin Kb1; Type-II keratin Kb8; UniProtKB:P04264}; UniProtKB:P05783}; UniProtKB:P13645}; UniProtKB:P19012}; unnamed protein product
• Gene Symbols: KRT10, Krt14, KRT15, KRT16, KRT18, KRT19, KRT9
• Host Species: Mouse
• Immunogen: Human epidermal keratins from P3 x 63 Ag8 cells.
• Purification Method: Protein G
• Quantity: 200 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100009029, 100126286, 100138169, 100341120, 100341376, 100354174, 100515339, 100515506, 100519971, 101749333, 107656, 16661, 16664, 16665, 16666, 16668, 16669, 266717, 281888, 287700, 287701, 294853, 303530, 360626, 3857, 3858, 3861, 3866, 3868, 3875, 3880, 395861, 404111, 408039, 408040, 450225, 506480, 507464, 514812, 771977, 788727
• Target Species: Human, Mouse, Rat, Rabbit, Monkey, Bovine, Pig, Chicken
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov