BioVision Inc D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric): D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric): A fast, sensitive, easy to use and HTP adaptable colorimetric assay to measure D2HG in various biological samples. 100 assays
Manufacturer: BioVision Inc K213100
D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric): In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of ?-ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. BioVision's D-2-Hydroxyglutarate Assay Kit provides a convenient method to detect D2HG in biological samples. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 ¿M in various samples.