Invitrogen™ DAAM1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA582927

Catalog No. PIPA582927

Description

Immunogen sequence: QNDKGQDPDS TPLENFNIKN VVRMLVNENE VKQWKEQAEK MRKEHNELQQ KLEKKERECD AKTQEKEEMM QTLNKMKEKL EKETTEHKQV KQQVADLTAQ LHELSRRAVC ASIPG.

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

Specifications

• Antigen: DAAM1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DAAM1
• Gene Alias: Daam1; Disheveled-associated activator of morphogenesis 1; dishevelled associated activator of morphogenesis 1; I79_021650; KIAA0666
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23002
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.6 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9Y4D1
• Gene Symbols: DAAM1
• Immunogen: Recombinant protein corresponding to Human DAAM1. Recombinant protein control fragment (Product #RP-89125).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov