Invitrogen™ DAN (PARN) Mouse ELISA Kit

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EMPARN	Invitrogen™ EMPARN	96 Tests
EMPARNX10	Invitrogen™ EMPARNX10	10 x 96 Tests
EMPARNX5	Invitrogen™ EMPARNX5	5 x 96 Tests

Description

The Mouse DAN (PARN) ELISA quantitates Ms DAN in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DAN. Principle of the method The Mouse DAN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a 3′-exoribonuclease, with similarity to the RNase D family of 3′-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: Q8VDG3
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: 1200003I18Rik,DAN
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 35 pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA pair antibody detects mouse DAN, no cross-reactivity with rhDAN, rhCOCO, rmBMP-4 or rmGremlin
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 74108
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Other Research
• Synonym: 1200003I18Rik, DAN
• Test Time: 1 hr. 20 min.