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Dendritic Cell Marker DCIR2 Rat anti-Mouse, PE, Clone: 33D1, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 12588483

Encompass

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Catalog No. 50-109-46


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Description

Description

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer′s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii. Applications Reported: The 33D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer′s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Specifications

Specifications

Dendritic Cell Marker DCIR2
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
DC Marker; DC inhibitor receptor 2; DCIR2
Rat
200 μg
Primary
Mouse
Antibody
IgG2b κ
Flow Cytometry
33D1
PE
Clec4a4
Clec4a4
Affinity chromatography
RUO
474145
4° C, store in dark, DO NOT FREEZE!
Liquid
SDS
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Product Certifications
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