DFFB Monoclonal Antibody (36A349), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA141140

Catalog No. 01-676-475

Description

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, or UV irradiation, anticancer drugs, and survival factor deprivation. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis.

Specifications

• Antigen: DFFB
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O54788, O76075
• Gene Symbols: DFFB
• Immunogen: Synthetic peptide corresponding to residues 205-222 of human DFF40.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: 36A349
• Conjugate: Unconjugated
• Gene: DFFB
• Gene Alias: 2410008J01Rik; 40kDa; 5730477D02Rik; AU018859; CAD; caspase-activated deoxyribonuclease; caspase-activated DNase; caspase-activated nuclease; Cpad; CPAN; DFF2; DFF-2; DFF40; DFF-40; DFFB; Didff; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase); DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, 40 kD, beta subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta subunit; DNase inhibited by DNA fragmentation factor; RP13-531C17.1; RP23-254N4.2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 13368, 1677
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid