R&D Systems™ Diaminobenzidine (DAB) Solution
R&D Systems™ Diaminobenzidine (DAB) Solution is a component of the reagent kit that is used for in situ detection of apoptosis in tissue sections and cells.
Manufacturer: R&D Systems™ 48003007
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Do not use past expiration date.
- Thaw at 37°C for 30 min.
- If not using the full bottle, store the remaining stock solution back at -20°C.
- It is not recommended to freeze thaw DAB stock solution more than three times.
- If required, aliquot in smaller volume and store at -20°C protected from light.
- It is recommended that 6mL aliquots of fresh 30% hydrogen peroxide are made and stored at 4°C.
- For each labeling procedure use a fresh 30% hydrogen peroxide aliquot then discard any remaining solution.
- Do not place on ice after thawing, otherwise the solution will precipitate.
- Prepare the solution no more than 30 minutes before use.
- Use 50mL of DAB Solution to process 1 to 10 samples.
|Store the unopened product at -70° C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.|
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